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PdnaK-IR3-IR3-Bxb1-CadR-pZa
(Plasmid #204686)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204686 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZa
  • Backbone size w/o insert (bp) 2093
  • Total vector size (bp) 3692
  • Modifications to backbone
    Addition of PdnaK promoter and IR3-IR3 operator sites.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Bxb1
  • Species
    Synthetic
  • Insert Size (bp)
    1127
  • Entrez Gene
    Bxb1p45 (a.k.a. Bxb1p45)
  • Promoter Inducible, HspR is its repressor
  • Tag / Fusion Protein
    • CadR (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PdnaK-IR3-IR3-Bxb1-CadR-pZa was a gift from Urartu Seker (Addgene plasmid # 204686 ; http://n2t.net/addgene:204686 ; RRID:Addgene_204686)

  • For your References section:

    A Recombinase-Based Genetic Circuit for Heavy Metal Monitoring. Akboga D, Saltepe B, Bozkurt EU, Seker UOS. Biosensors (Basel). 2022 Feb 16;12(2):122. doi: 10.3390/bios12020122. 10.3390/bios12020122 PubMed 35200383
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