UCK2_Deletion_gRNA_1
(Plasmid
#204672)
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PurposeDual gRNA plasmid for UCK2 deletion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204672 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLentiV_sgRNA_Cas9_GFP
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Vector typeLentiviral
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameuridine-cytidine kinase 2
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gRNA/shRNA sequenceATGAAGTTCAGGTTGTACGA ; TTAGAAGTTAGCGAGCCCAG
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SpeciesH. sapiens (human)
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Entrez GeneUCK2 (a.k.a. TSA903, UK, UMPK)
- Promoter U6
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UCK2_Deletion_gRNA_1 was a gift from Jason Sheltzer (Addgene plasmid # 204672 ; http://n2t.net/addgene:204672 ; RRID:Addgene_204672)