Skip to main content
Addgene

U6-AsCas12f-full-length-sgRNA
(Plasmid #204638)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204638 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    de novo assembled
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    full-length AsCas12f sgRNA
  • gRNA/shRNA sequence
    AGGCATCACTGCCCCCTGAT
  • Species
    Synthetic
  • Promoter U6

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6-AsCas12f-full-length-sgRNA was a gift from Weixin Tang (Addgene plasmid # 204638 ; http://n2t.net/addgene:204638 ; RRID:Addgene_204638)

  • For your References section:

    An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity. Wu T, Liu C, Zou S, Lyu R, Yang B, Yan H, Zhao M, Tang W. Nat Chem Biol. 2023 Jul 3. doi: 10.1038/s41589-023-01380-9. 10.1038/s41589-023-01380-9 PubMed 37400536
Related items:
Commonly requested with: