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Addgene

CMV-AsCas12f-v4.1
(Plasmid #204636)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204636 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    de novo assembled
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AsCas12f-v4.1
  • Alt name
    Cas12U3
  • Species
    Synthetic
  • Mutation
    D169K, N199K, N328G, D364R
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-AsCas12f-v4.1 was a gift from Weixin Tang (Addgene plasmid # 204636 ; http://n2t.net/addgene:204636 ; RRID:Addgene_204636)

  • For your References section:

    An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity. Wu T, Liu C, Zou S, Lyu R, Yang B, Yan H, Zhao M, Tang W. Nat Chem Biol. 2023 Jul 3. doi: 10.1038/s41589-023-01380-9. 10.1038/s41589-023-01380-9 PubMed 37400536
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