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pET-15b-aδNb072
(Plasmid #204627)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204627 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5645
  • Total vector size (bp) 6146
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    aδNb072
  • Alt name
    anti-IgD nanobody 072
  • Species
    Synthetic
  • Insert Size (bp)
    501
  • Promoter T7
  • Tags / Fusion Proteins
    • TEV protease cleavage site (C terminal on insert)
    • His6 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-15b-aδNb072 was a gift from James McDonnell (Addgene plasmid # 204627 ; http://n2t.net/addgene:204627 ; RRID:Addgene_204627)

  • For your References section:

    Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor. Vester SK, Davies AM, Beavil RL, Sandhar BS, Beavil AJ, Gould HJ, Sutton BJ, McDonnell JM. Antibodies (Basel). 2023 Nov 16;12(4):75. doi: 10.3390/antib12040075. 10.3390/antib12040075 PubMed 37987253
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