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pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA
(Plasmid #204620)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204620 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPV
  • Backbone size w/o insert (bp) 5460
  • Vector type
    CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    crRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)
  • gRNA/shRNA sequence
    crRNA(DMD#20): ctgacagtagaccccagtacatgcttcctaaa, crRNA(DMD#23): tcttggtgagaatcatattctgtagtacaagg
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    240
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer GACTATCATATGCTTACCGT
  • 3′ sequencing primer gatcgctctagactagtggatc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA was a gift from Akitsu Hotta (Addgene plasmid # 204620 ; http://n2t.net/addgene:204620 ; RRID:Addgene_204620)

  • For your References section:

    Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs. Kita Y, Okuzaki Y, Naoe Y, Lee J, Bang U, Okawa N, Ichiki A, Jonouchi T, Sakurai H, Kojima Y, Hotta A. Stem Cell Reports. 2023 Sep 12;18(9):1753-1765. doi: 10.1016/j.stemcr.2023.07.007. Epub 2023 Aug 24. 10.1016/j.stemcr.2023.07.007 PubMed 37625413
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