Skip to main content
Addgene

pFPV25-cR-2iG
(Plasmid #204618)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204618 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFPV25
  • Total vector size (bp) 7644
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    rpsM promoter
  • Species
    Salmonella enterica
  • Insert Size (bp)
    613
  • Tag / Fusion Protein
    • dsRed

Gene/Insert 2

  • Gene/Insert name
    sseA promoter
  • Species
    Salmonella enterica
  • Insert Size (bp)
    1505
  • Tag / Fusion Protein
    • GFP

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

- The ampicillin resistance cassette was changed to a kanamycin resistance cassette.
- dsRed is constitutively expressed under the PrpsM Salmonella promoter.
- GFP is expressed under the PsseA Salmonella SPI2 promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFPV25-cR-2iG was a gift from Jacques Neefjes (Addgene plasmid # 204618 ; http://n2t.net/addgene:204618 ; RRID:Addgene_204618)

  • For your References section:

    Multi-omics analyses of cancer-linked clinical salmonellae reveal bacterial-induced host metabolic shift and mTOR-dependent cell transformation. Stévenin V, Coipan CE, Duijster JW, van Elsland DM, Voogd L, Bigey L, van Hoek AHAM, Wijnands LM, Janssen L, Akkermans JJLL, Neefjes-Borst A, Franz E, Mughini-Gras L, Neefjes J. Cell Rep. 2024 Nov 1;43(11):114931. doi: 10.1016/j.celrep.2024.114931. 10.1016/j.celrep.2024.114931 PubMed 39488829
Related items: