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Addgene

pMYCO1_SpdCas9_pmcDA1
(Plasmid #204615)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204615 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMYCO1
  • Vector type
    Bacterial Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMYCO1_SpdCas9_pmcDA1 was a gift from Pascal Sirand-Pugnet (Addgene plasmid # 204615 ; http://n2t.net/addgene:204615 ; RRID:Addgene_204615)

  • For your References section:

    Genome Editing of Veterinary Relevant Mycoplasmas Using a CRISPR-Cas Base Editor System. Ipoutcha T, Rideau F, Gourgues G, Arfi Y, Lartigue C, Blanchard A, Sirand-Pugnet P. Appl Environ Microbiol. 2022 Sep 13;88(17):e0099622. doi: 10.1128/aem.00996-22. Epub 2022 Aug 24. 10.1128/aem.00996-22 PubMed 36000854
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