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Addgene

ACBE
(Plasmid #204607)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204607 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
  • Insert Size (bp)
    6726
  • Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
  • Tag / Fusion Protein
    • 6*His (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ACBE was a gift from Dali Li (Addgene plasmid # 204607 ; http://n2t.net/addgene:204607 ; RRID:Addgene_204607)

  • For your References section:

    Adenine transversion editors enable precise, efficient A*T-to-C*G base editing in mammalian cells and embryos. Chen L, Hong M, Luan C, Gao H, Ru G, Guo X, Zhang D, Zhang S, Li C, Wu J, Randolph PB, Sousa AA, Qu C, Zhu Y, Guan Y, Wang L, Liu M, Feng B, Song G, Liu DR, Li D. Nat Biotechnol. 2023 Jun 15. doi: 10.1038/s41587-023-01821-9. 10.1038/s41587-023-01821-9 PubMed 37322276
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