AXBEv2
(Plasmid
#204606)
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PurposeMammalian expression of evolved adenine transversion base editor AXBEv2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204606 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
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Insert Size (bp)6726
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MutationmAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
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Tag
/ Fusion Protein
- 6*His (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-Forward
- 3′ sequencing primer BGH-Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AXBEv2 was a gift from Dali Li (Addgene plasmid # 204606 ; http://n2t.net/addgene:204606 ; RRID:Addgene_204606) -
For your References section:
Adenine transversion editors enable precise, efficient A*T-to-C*G base editing in mammalian cells and embryos. Chen L, Hong M, Luan C, Gao H, Ru G, Guo X, Zhang D, Zhang S, Li C, Wu J, Randolph PB, Sousa AA, Qu C, Zhu Y, Guan Y, Wang L, Liu M, Feng B, Song G, Liu DR, Li D. Nat Biotechnol. 2023 Jun 15. doi: 10.1038/s41587-023-01821-9. 10.1038/s41587-023-01821-9 PubMed 37322276