pBV90
(Plasmid #204601)

• Purpose: Expresses OsTir1-3X Myc from S. cerevisiae ADH1 promoter
• Depositing Lab: Scott Moye-Rowley
• Publication: Plasmids for auxin-inducible degron tagging of genes in Candida glabrata (unpublished)

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 9083
• Vector type: Bacterial Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Os Tir1
• Alt name: transport inhibitor response
• Species: Oryza sativa
• Insert Size (bp): 6430
• GenBank ID: XP_015635915.1
• Entrez Gene: LOC4338955 (a.k.a. OSNPB_050449500, B1122D01.5, COI1B, OsCOI1H, OsCOI1b, OsJ_18742)
• Promoter: ScADH1
• Tag / Fusion Protein:
  • Myc epitope tags

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CAAGGCGATTAAGTTGGGTAAC

Resource Information

• Supplemental Documents:
  • pBV90_pUC19-LEU2-OsTir1-9Myc into TRP1 locus.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry