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Addgene

pACS71
(Plasmid #204588)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204588 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4t1
  • Backbone size w/o insert (bp) 4939
  • Total vector size (bp) 5775
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human lamin A (389-597)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    836
  • GenBank ID
    4000
  • Tag / Fusion Protein
    • GST-lamin A (389-597) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACS71 was a gift from Tom Misteli (Addgene plasmid # 204588 ; http://n2t.net/addgene:204588 ; RRID:Addgene_204588)

  • For your References section:

    Identification of epigenetic modulators as determinants of nuclear size and shape. Schibler AC, Jevtic P, Pegoraro G, Levy DL, Misteli T. Elife. 2023 May 23;12:e80653. doi: 10.7554/eLife.80653. 10.7554/eLife.80653 PubMed 37219077
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