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Addgene

p3xFLAG-p62 PB1mut
(Plasmid #204577)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204577 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3_FLAG-CMV14
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p62
  • Species
    M. musculus (mouse)
  • Mutation
    K7A/D69A
  • Entrez Gene
    Sqstm1 (a.k.a. A170, OSF-6, Osi, STAP, STONE14, p62)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-p62 PB1mut was a gift from Masaaki Komatsu (Addgene plasmid # 204577 ; http://n2t.net/addgene:204577 ; RRID:Addgene_204577)

  • For your References section:

    Negative regulation of the Keap1-Nrf2 pathway by a p62/Sqstm1 splicing variant. Kageyama S, Saito T, Obata M, Koide RH, Ichimura Y, Komatsu M. Mol Cell Biol. 2018 Jan 16. pii: MCB.00642-17. doi: 10.1128/MCB.00642-17. 10.1128/MCB.00642-17 PubMed 29339380
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