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Addgene

pHis-3xFLAG-CMV14-CYB5R3
(Plasmid #204551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3_FLAG-CMV14
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CYB5R3
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Entrez Gene
    CYB5R3 (a.k.a. B5R, DIA1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHis-3xFLAG-CMV14-CYB5R3 was a gift from Masaaki Komatsu (Addgene plasmid # 204551 ; http://n2t.net/addgene:204551 ; RRID:Addgene_204551)

  • For your References section:

    The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3. Ishimura R, El-Gowily AH, Noshiro D, Komatsu-Hirota S, Ono Y, Shindo M, Hatta T, Abe M, Uemura T, Lee-Okada HC, Mohamed TM, Yokomizo T, Ueno T, Sakimura K, Natsume T, Sorimachi H, Inada T, Waguri S, Noda NN, Komatsu M. Nat Commun. 2022 Dec 21;13(1):7857. doi: 10.1038/s41467-022-35501-0. 10.1038/s41467-022-35501-0 PubMed 36543799
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