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pRetroX-TetOne-Puro-p62 deltaUBA
(Plasmid #204550)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204550 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetroX-TetOne-Puro
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p62
  • Alt name
    SQSTM1
  • Species
    H. sapiens (human)
  • Mutation
    deltaUBA
  • Entrez Gene
    SQSTM1 (a.k.a. A170, DMRV, EBIAP, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroX-TetOne-Puro-p62 deltaUBA was a gift from Masaaki Komatsu (Addgene plasmid # 204550 ; http://n2t.net/addgene:204550 ; RRID:Addgene_204550)

  • For your References section:

    Integrated proteomics identifies p62-dependent selective autophagy of the supramolecular vault complex. Kurusu R, Fujimoto Y, Morishita H, Noshiro D, Takada S, Yamano K, Tanaka H, Arai R, Kageyama S, Funakoshi T, Komatsu-Hirota S, Taka H, Kazuno S, Miura Y, Koike M, Wakai T, Waguri S, Noda NN, Komatsu M. Dev Cell. 2023 May 9:S1534-5807(23)00191-0. doi: 10.1016/j.devcel.2023.04.015. 10.1016/j.devcel.2023.04.015 PubMed 37192622
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