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pLKO.3R/MPL-shRNA2
(Plasmid #204527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204527 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.3R
  • Backbone manufacturer
    Christophe Benoist & Diane Mathis (Addgene plasmid # 14748)
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MPL-shRNA2
  • gRNA/shRNA sequence
    CCGGGCCCAAGAGACCTGTTATCAACTCGAGTTGATAACAGGTCTCTTGGGCTTTTTG
  • Species
    H. sapiens (human)
  • Entrez Gene
    MPL (a.k.a. C-MPL, CD110, MPLV, THCYT2, THPOR, TPOR)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.3R/MPL-shRNA2 was a gift from Norio Komatsu (Addgene plasmid # 204527 ; http://n2t.net/addgene:204527 ; RRID:Addgene_204527)

  • For your References section:

    Activation of the thrombopoietin receptor by mutant calreticulin in CALR-mutant myeloproliferative neoplasms. Araki M, Yang Y, Masubuchi N, Hironaka Y, Takei H, Morishita S, Mizukami Y, Kan S, Shirane S, Edahiro Y, Sunami Y, Ohsaka A, Komatsu N. Blood. 2016 Jan 27. pii: blood-2015-09-671172. 10.1182/blood-2015-09-671172 PubMed 26817954
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