pMSCV-IRES-GFP/CALR ins5-FLAG
(Plasmid
#204524)
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PurposeRetroviral expression of human CALR ins5-FLAG
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204524 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMSCV-IRES-GFP
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Backbone manufacturerTannishtha Reya (Addgene plasmid # 20672)
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCALR ins5-FLAG
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SpeciesH. sapiens (human)
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Mutationins5
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Entrez GeneCALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-IRES-GFP/CALR ins5-FLAG was a gift from Norio Komatsu (Addgene plasmid # 204524 ; http://n2t.net/addgene:204524 ; RRID:Addgene_204524) -
For your References section:
Activation of the thrombopoietin receptor by mutant calreticulin in CALR-mutant myeloproliferative neoplasms. Araki M, Yang Y, Masubuchi N, Hironaka Y, Takei H, Morishita S, Mizukami Y, Kan S, Shirane S, Edahiro Y, Sunami Y, Ohsaka A, Komatsu N. Blood. 2016 Jan 27. pii: blood-2015-09-671172. 10.1182/blood-2015-09-671172 PubMed 26817954