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Addgene

pHIT239
(Plasmid #204522)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204522 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHIT88 (derived from pCITE-4b)
  • Backbone size w/o insert (bp) 3073
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mos1_transposase::glh-2_3'UTR
  • Species
    C. elegans (nematode)
  • Entrez Gene
    glh-2
  • Promoter T7 (minimum seq.)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site StuI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer f1 origin, reverse primer
  • 3′ sequencing primer T7 terminator, reverse primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T7p, SL1, StuI, Mos1 transposase, NheI, glh-2 3’UTR, XbaI, a 61-nt poly(A), BsaI and SpeI; linearize with BsaI and use for in vitro transcription of mRNA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIT239 was a gift from Yuji Kohara (Addgene plasmid # 204522 ; http://n2t.net/addgene:204522 ; RRID:Addgene_204522)

  • For your References section:

    A small RNA system ensures accurate homologous pairing and unpaired silencing of meiotic chromosomes. Tabara H, Mitani S, Mochizuki M, Kohara Y, Nagata K. EMBO J. 2023 Jun 1;42(11):e105002. doi: 10.15252/embj.2020105002. Epub 2023 Apr 20. 10.15252/embj.2020105002 PubMed 37078421
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