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Addgene

pHIT521
(Plasmid #204520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHIT198 (derived from pET-28a)
  • Backbone size w/o insert (bp) 6442
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    N-terminal fragment of cec-5 (C. elegans)
  • Species
    C. elegans (nematode)
  • Promoter T7lac
  • Tag / Fusion Protein
    • 6xHis, MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer MBP_sense
  • 3′ sequencing primer T7 terminator, reverse primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The C-terminal end of the fusion protein might be partially sensitive to bacterial proteases

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIT521 was a gift from Yuji Kohara (Addgene plasmid # 204520 ; http://n2t.net/addgene:204520 ; RRID:Addgene_204520)

  • For your References section:

    A small RNA system ensures accurate homologous pairing and unpaired silencing of meiotic chromosomes. Tabara H, Mitani S, Mochizuki M, Kohara Y, Nagata K. EMBO J. 2023 Jun 1;42(11):e105002. doi: 10.15252/embj.2020105002. Epub 2023 Apr 20. 10.15252/embj.2020105002 PubMed 37078421
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