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Addgene

pHIT182
(Plasmid #204511)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204511 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHIT198 (derived from pET-28a)
  • Backbone size w/o insert (bp) 6442
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    csr-1a with D769A mutation (C. elegans)
  • Species
    C. elegans (nematode)
  • Mutation
    D769A
  • Entrez Gene
    csr-1 (a.k.a. CELE_F20D12.1)
  • Promoter T7lac
  • Tag / Fusion Protein
    • 6xHis, MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site StuI (unknown if destroyed)
  • 5′ sequencing primer MBP_sense
  • 3′ sequencing primer T7 terminator, reverse primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIT182 was a gift from Yuji Kohara (Addgene plasmid # 204511 ; http://n2t.net/addgene:204511 ; RRID:Addgene_204511)

  • For your References section:

    In vitro analyses of the production and activity of secondary small interfering RNAs in C. elegans. Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H. EMBO J. 2007 Dec 12. 26(24):5007-19. 10.1038/sj.emboj.7601910 PubMed 18007599
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