pHIT179
(Plasmid
#204510)
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PurposeBacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204510 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHIT198 (derived from pET-28a)
- Backbone size w/o insert (bp) 6442
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namecsr-1a (C. elegans)
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SpeciesC. elegans (nematode)
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Entrez Genecsr-1 (a.k.a. CELE_F20D12.1)
- Promoter T7lac
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Tag
/ Fusion Protein
- 6xHis, MBP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site StuI (unknown if destroyed)
- 5′ sequencing primer MBP_sense
- 3′ sequencing primer T7 terminator, reverse primer (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHIT179 was a gift from Yuji Kohara (Addgene plasmid # 204510 ; http://n2t.net/addgene:204510 ; RRID:Addgene_204510) -
For your References section:
In vitro analyses of the production and activity of secondary small interfering RNAs in C. elegans. Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H. EMBO J. 2007 Dec 12. 26(24):5007-19. 10.1038/sj.emboj.7601910 PubMed 18007599