pHIT198
(Plasmid
#204509)
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Purpose(Empty Backbone) pET-derived vector for expression of proteins N-terminally tagged with 6xHis and maltose binding protein (multi-cloning sites: NdeI, NotI, SpeI, SalI, PstI, SgfI, and XhoI)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204509 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-28a
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Backbone manufacturerNovagen
- Backbone size (bp) 6442
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- 6xHis, MBP (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- 6xHis, MBP (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHIT198 was a gift from Yuji Kohara (Addgene plasmid # 204509 ; http://n2t.net/addgene:204509 ; RRID:Addgene_204509) -
For your References section:
In vitro analyses of the production and activity of secondary small interfering RNAs in C. elegans. Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H. EMBO J. 2007 Dec 12. 26(24):5007-19. 10.1038/sj.emboj.7601910 PubMed 18007599