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Addgene

pHIT198
(Plasmid #204509)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204509 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET-28a
  • Backbone manufacturer
    Novagen
  • Backbone size (bp) 6442
  • Vector type
    Bacterial Expression
  • Tag / Fusion Protein
    • 6xHis, MBP (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • 6xHis, MBP (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIT198 was a gift from Yuji Kohara (Addgene plasmid # 204509 ; http://n2t.net/addgene:204509 ; RRID:Addgene_204509)
  • For your References section:

    In vitro analyses of the production and activity of secondary small interfering RNAs in C. elegans. Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H. EMBO J. 2007 Dec 12. 26(24):5007-19. 10.1038/sj.emboj.7601910 PubMed 18007599