aCE120
(Plasmid
#204491)
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PurposeFigure 1. reporter circuit
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 204491 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneplasmid DNA
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Vector typeMammalian Expression, Bacterial Expression, Synthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameEBFP2
- Promoter hEF1a
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namemKate2
- Promoter hEF1a
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Tags
/ Fusion Proteins
- none
- none
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert nameEYFP
- Promoter hEF1a
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Tags
/ Fusion Proteins
- none
- none
Cloning Information for Gene/Insert 3
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
aCE120 was a gift from Ron Weiss (Addgene plasmid # 204491 ; http://n2t.net/addgene:204491 ; RRID:Addgene_204491) -
For your References section:
Synthetic symmetry breaking and programmable multicellular structure formation. Wauford N, Patel A, Tordoff J, Enghuus C, Jin A, Toppen J, Kemp ML, Weiss R. Cell Syst. 2023 Sep 20;14(9):806-818.e5. doi: 10.1016/j.cels.2023.08.001. Epub 2023 Sep 8. 10.1016/j.cels.2023.08.001 PubMed 37689062