pAAV-sL7-Cre-HA-WPRE
(Plasmid #204488)

• Purpose: For production of driver AAV virus for Purkinje cell-specific expression
• Depositing Lab: Samuel Wang
• Publication: Chen et al Nature. 2022 May;605(7911):722-727. doi: 10.1038/s41586-022-04711-3. Epub 2022 May 11.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5186
• Total vector size (bp): 6212
• Vector type: Mammalian Expression, Mouse Targeting, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cre
• Species: Synthetic
• Insert Size (bp): 1026
• Promoter: sL7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CATGTTGGTTGATCTTCAAC
• 3′ sequencing primer: AATCAACCTCTGGATTACAA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry