PEmax-mRNA
(Plasmid #204472)

• Purpose: plasmid for PEmax mRNA IVT
• Depositing Lab: Scot Wolfe
• Publication: Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456. doi: 10.1093/nar/gkad456.

Backbone

• Vector backbone: pbluescript
• Backbone manufacturer: NA
• Backbone size w/o insert (bp): 3300
• Total vector size (bp): 9167
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Prime editor
• Alt name: PEmax mRNA
• Species: human
• Promoter: T7
• Tag / Fusion Protein:
  • no

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 Rev
• 3′ sequencing primer: polyA-seq

Resource Information

• Supplemental Documents:
  • PEmax-mRNA.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry