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Addgene

PE-Max-pet21A
(Plasmid #204471)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204471 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet21A
  • Backbone manufacturer
    NA
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 11907
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Prime editor
  • Alt name
    PEmax
  • Species
    bacteria
  • Insert Size (bp)
    6499
  • Tag / Fusion Protein
    • His-tag (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PE-Max-pet21A was a gift from Scot Wolfe (Addgene plasmid # 204471 ; http://n2t.net/addgene:204471 ; RRID:Addgene_204471)

  • For your References section:

    Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiency. Ponnienselvan K, Liu P, Nyalile T, Oikemus S, Maitland SA, Lawson ND, Luban J, Wolfe SA. Nucleic Acids Res. 2023 May 29:gkad456. doi: 10.1093/nar/gkad456. 10.1093/nar/gkad456 PubMed 37246708
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