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Addgene

wt Opa1
(Plasmid #204427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hOpa1 isoform 1
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Entrez Gene
    OPA1 (a.k.a. BERHS, MGM1, MTDPS14, NPG, NTG, largeG)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CNV-F
  • 3′ sequencing primer SV40-pArev
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    wt Opa1 was a gift from Keir Neuman (Addgene plasmid # 204427 ; http://n2t.net/addgene:204427 ; RRID:Addgene_204427)

  • For your References section:

    OPA1 helical structures give perspective to mitochondrial dysfunction. Nyenhuis SB, Wu X, Strub MP, Yim YI, Stanton AE, Baena V, Syed ZA, Canagarajah B, Hammer JA, Hinshaw JE. Nature. 2023 Aug 23. doi: 10.1038/s41586-023-06462-1. 10.1038/s41586-023-06462-1 PubMed 37612506
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