anti-PSD-95 scFv [K28/43] with sortase tag
(Plasmid
#204422)
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PurposeMammalian Expression of anti-PSD-95 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K28/43.
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.4
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Backbone manufacturerInvitrogen
- Total vector size (bp) 6011
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRecombinant mouse scFv targeting PSD-95 (Homo sapiens)
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SpeciesM. musculus (mouse)
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Insert Size (bp)852
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Tags
/ Fusion Proteins
- Sortase tag (C terminal on insert)
- 6xHis tag (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
anti-PSD-95 scFv [K28/43] with sortase tag was a gift from Jeff Lichtman (Addgene plasmid # 204422 ; http://n2t.net/addgene:204422 ; RRID:Addgene_204422) -
For your References section:
Multiplexed volumetric CLEM enabled by antibody derivatives provides new insights into the cytology of the mouse cerebellar cortex. Han X, Lu X, Li PH, Wang S, Schalek R, Meirovitch Y, Lin Z, Adhinarta J, Berger D, Wu Y, Fang T, Meral ES, Asraf S, Ploegh H, Pfister H, Wei D, Jain V, Trimmer JS, Lichtman JW. bioRxiv. 2023 Jun 8:2023.05.20.540091. doi: 10.1101/2023.05.20.540091. Preprint. 10.1101/2023.05.20.540091 PubMed 37292964