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Addgene

pRK5-HA-YFP-Raptor K840R
(Plasmid #204418)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204418 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4754
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Regulatory Associated Protein Of MTOR, Complex 1
  • Alt name
    raptor
  • Species
    H. sapiens (human)
  • Mutation
    Lysine 840 to Arginine (K840R)
  • GenBank ID
    NM_020761
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • YFP (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mutagensis of pRK5-HA-YFP-raptor (Addgene #73385)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-HA-YFP-Raptor K840R was a gift from David Rubinsztein (Addgene plasmid # 204418 ; http://n2t.net/addgene:204418 ; RRID:Addgene_204418)

  • For your References section:

    Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A. Son SM, Park SJ, Lee H, Siddiqi F, Lee JE, Menzies FM, Rubinsztein DC. Cell Metab. 2019 Jan 8;29(1):192-201.e7. doi: 10.1016/j.cmet.2018.08.013. Epub 2018 Sep 6. 10.1016/j.cmet.2018.08.013 PubMed 30197302
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