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Addgene

pET28C-mCherry-SARS-CoV-2-Nucleocapsid
(Plasmid #204403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28C(+)
  • Backbone manufacturer
    Novagen (EMD Millipore)
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 nucleocapsid
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    1979
  • Entrez Gene
    N (a.k.a. GU280_gp10)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x-His; mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene #141391
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28C-mCherry-SARS-CoV-2-Nucleocapsid was a gift from Samie Jaffrey (Addgene plasmid # 204403 ; http://n2t.net/addgene:204403 ; RRID:Addgene_204403)

  • For your References section:

    Biomolecular condensates create phospholipid-enriched microenvironments. Dumelie JG, Chen Q, Miller D, Attarwala N, Gross SS, Jaffrey SR. Nat Chem Biol. 2023 Nov 16. doi: 10.1038/s41589-023-01474-4. 10.1038/s41589-023-01474-4 PubMed 37973889
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