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Addgene

hKCNQ5(P369T).ires.GFP-pcDNA3
(Plasmid #204365)

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Item Catalog # Description Quantity Price (USD)
Plasmid 204365 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KCNQ5
  • Species
    H. sapiens (human)
  • Mutation
    P369T
  • GenBank ID
    AF249278.1
  • Entrez Gene
    KCNQ5 (a.k.a. Kv7.5, MRD46)
  • Promoter CMV/T7 RNApol

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutation created in KCNQ5-202 splice variant (Ensembl), referenced to KCNQ5-201 (Ensembl).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hKCNQ5(P369T).ires.GFP-pcDNA3 was a gift from Aguan Wei (Addgene plasmid # 204365 ; http://n2t.net/addgene:204365 ; RRID:Addgene_204365)

  • For your References section:

    Human KCNQ5 de novo mutations underlie epilepsy and intellectual disability. Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, Ramirez JM, Kalume FK. J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021. Epub 2022 May 18. 10.1152/jn.00509.2021 PubMed 35583973
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