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Addgene

pLenti_PGK_Prkaa1
(Plasmid #204356)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204356 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti PGK Puro
  • Backbone manufacturer
    #19068 modified
  • Backbone size w/o insert (bp) 7951
  • Total vector size (bp) 9631
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AMP-activated protein kinase alpha 1
  • Alt name
    Prkaa1
  • Alt name
    AMPKa1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1680
  • GenBank ID
    NM_001013367.3
  • Entrez Gene
    Prkaa1 (a.k.a. AMPKalpha1, C130083N04Rik)
  • Promoter PGK

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GTGTTCCGCATTCTGCAAG
  • 3′ sequencing primer CATAGCGTAAAAGGAGCAACA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_PGK_Prkaa1 was a gift from Günter Schneider (Addgene plasmid # 204356 ; http://n2t.net/addgene:204356 ; RRID:Addgene_204356)
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