Skip to main content
Addgene

pB-TopFlash-NanoLucP
(Plasmid #204347)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204347 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    piggybac
  • Backbone manufacturer
    n/a
  • Total vector size (bp) 9357
  • Vector type
    Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TopFlash-NanoLucP
  • Alt name
    TopFlash
  • Species
    Synthetic
  • Promoter miniP
  • Tag / Fusion Protein
    • NanoLucP

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATCCCGTGCCACCTTC
  • 3′ sequencing primer ctctaggcaccggttcaat
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-TopFlash-NanoLucP was a gift from Li-Fang Chu (Addgene plasmid # 204347 ; http://n2t.net/addgene:204347 ; RRID:Addgene_204347)

  • For your References section:

    Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing. Conrad JV, Meyer S, Ramesh PS, Neira JA, Rusteika M, Mamott D, Duffin B, Bautista M, Zhang J, Hiles E, Higgins EM, Steill J, Freeman J, Ni Z, Liu S, Ungrin M, Rancourt D, Clegg DO, Stewart R, Thomson JA, Chu LF. Stem Cell Reports. 2023 Dec 12;18(12):2328-2343. doi: 10.1016/j.stemcr.2023.10.009. Epub 2023 Nov 9. 10.1016/j.stemcr.2023.10.009 PubMed 37949072
Related items:
Commonly requested with: