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Addgene

pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO
(Plasmid #204342)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204342 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-IRES-Hygro
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hSyn Galpha-s/Gbetagamma ONE-GO
  • Alt name
    GNAS/GNB1/GNG2
  • Species
    GNAS = H. sapiens (human) ; GNB1/GNG2 = H. sapiens (human)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO was a gift from Mikel Garcia-Marcos (Addgene plasmid # 204342 ; http://n2t.net/addgene:204342 ; RRID:Addgene_204342)

  • For your References section:

    Direct interrogation of context-dependent GPCR activity with a universal biosensor platform. Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M. Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028. 10.1016/j.cell.2024.01.028 PubMed 38412860
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