pJAT18
(Plasmid #204307)

• Purpose: For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.
• Depositing Lab: David Stern
• Publication: Stern et al bioRxiv 2023.06.17.545412

Backbone

• Vector backbone: Janelia Atalanta cloning plasmid
• Vector type: CRISPR
• Selectable markers: ccdB, SacB

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Spectinomycin is in the backbone. Chlor and Amp are in the Gateway cloning arms. The plasmids can be maintained on any single of these antibiotics. Depositor typically grows them in Amp or Spec.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MHC-GFPn
• Alt name: pJAT18{dU6-3-attR3-Amp-ccdB-attR4-MHC-GFPn-attR5-Chlor-SacB-attR6}
• Species: Synthetic
• Promoter: MHC

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • pJAT18 GenBank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.06.17.545412v1 for bioRxiv preprint.