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pJAT17
(Plasmid #204298)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204298 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Janelia Atalanta cloning plasmid
  • Vector type
    CRISPR
  • Selectable markers
    ccdB, SacB

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Spectinomycin is in the backbone. Chlor and Amp are in the Gateway cloning arms. The plasmids can be maintained on any single of these antibiotics. Depositor typically grows them in Amp or Spec.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3XP3-EYFP
  • Alt name
    pJAT17{dU6-3-attR3-Amp-ccdB-attR4-nos-Cas9-nos3'UTR,3XP3-EYFP-attR5-Chlor-SacB-attR6}
  • Species
    Synthetic
  • Promoter 3XP3

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJAT17 was a gift from David Stern (Addgene plasmid # 204298 ; http://n2t.net/addgene:204298 ; RRID:Addgene_204298)

  • For your References section:

    The Janelia Atalanta plasmids provide a simple and efficient CRISPR/Cas9-mediated homology directed repair platform for Drosophila. Stern D, Kim E, Behrman E. bioRxiv 2023.06.17.545412 10.1101/2023.06.17.545412
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