pJAT32
(Plasmid
#204297)
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Purpose(Empty Backbone) pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204297 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneJanelia Atalanta cloning plasmid
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Vector typeCRISPR
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Selectable markersccdB, SacB
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsSpectinomycin is in the backbone. Chlor and Amp are in the Gateway cloning arms. The plasmids can be maintained on any single of these antibiotics. Depositor typically grows them in Amp or Spec.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepJAT32{dU6-3-attR3-Amp-ccdB-attR4-MCS-attR5-Chlor-SacB-attR6-dU6-1}
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.06.17.545412v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJAT32 was a gift from David Stern (Addgene plasmid # 204297 ; http://n2t.net/addgene:204297 ; RRID:Addgene_204297) -
For your References section:
The Janelia Atalanta plasmids provide a simple and efficient CRISPR/Cas9-mediated homology directed repair platform for Drosophila. Stern D, Kim E, Behrman E. bioRxiv 2023.06.17.545412 10.1101/2023.06.17.545412
