pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
(Plasmid #204288)

• Purpose: yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF
• Depositing Lab: Linda Huang
• Publication: Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. doi: 10.1091/mbc.E23-03-0096. Epub 2023 Jul 12.

Backbone

• Vector backbone: pHIS3p:yomRUBY2-TUB1+3’UTR from Markus et al., 2015 Traffic 16: 773-786
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
• Alt name: yomRuby3-Tub1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2795
• Promoter: pHis3
• Tag / Fusion Protein:
  • mRuby3 (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tagcgattggcattatcac
• 3′ sequencing primer: SP6

Resource Information

• Supplemental Documents:
  • pHIS3p-yomRUBY3-TUB1+3'UTR-LEU2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone is from Markus, et al., (2015) Traffice 16: 773-786. We swapped the marker and added in yomRuby3, which we had synthesized.