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Addgene

pQE60 P123G wgMDH
(Plasmid #204256)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204256 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE60
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 3391
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MDHG_CITLA
  • Alt name
    watermelon glyoxysomal malate dehydrogenase
  • Species
    Citrullus lanatus subsp. Vulgaris
  • Insert Size (bp)
    987
  • Mutation
    P123G
  • Tag / Fusion Protein
    • 6X His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CCCGAAAAGTGCCACCTG
  • 3′ sequencing primer CCAGTAATGACCTCAGAAC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Received from Leonard J. Banaszak; Original construct from Christine Gietl

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE60 P123G wgMDH was a gift from Malate dehydrogenase CUREs Community (MCC) (Addgene plasmid # 204256 ; http://n2t.net/addgene:204256 ; RRID:Addgene_204256)

  • For your References section:

    Organelle and translocatable forms of glyoxysomal malate dehydrogenase. The effect of the N-terminal presequence. Cox B, Chit MM, Weaver T, Gietl C, Bailey J, Bell E, Banaszak L. FEBS J. 2005 Feb;272(3):643-54. doi: 10.1111/j.1742-4658.2004.04475.x. 10.1111/j.1742-4658.2004.04475.x PubMed 15670147
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