pQE60 MGH
(Plasmid #204244)

• Purpose: Bacterial expression of a fusion of watermelon glyoxysomal malate dehydrogenase (MDH) and GFP with His tag; Use in CURES (integration of research into undergraduate teaching labs).
• Depositing Lab: Malate dehydrogenase CUREs Community (MCC)
• Publication: Expression of His tagged recombinant malate dehydrogenase (MDH) (unpublished)

Backbone

• Vector backbone: pQE60
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 3995
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MGH
• Alt name: MDH GFP His
• Alt name: watermelon MDH-GFP fusion
• Species: Citrullus lanatus subsp. Vulgaris
• Insert Size (bp): 1707
• Mutation: fusion of MDH and GFP with His tag
• GenBank ID: M33148.1
• Promoter: Lac
• Tags / Fusion Proteins:
  • GFP (C terminal on insert)
  • 6X His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (destroyed during cloning)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: CCCGAAAAGTGCCACCTG
• 3′ sequencing primer: CCAGTAATGACCTCAGAAC

Resource Information

• Supplemental Documents:
  • pQE60_MGH SnapGene
  • MCC Clone Information Sheet - MGH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/