pET24a MDH_9CREN
(Plasmid #204243)

• Purpose: Bacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
• Depositing Lab: Malate dehydrogenase CUREs Community (MCC)
• Publication: Expression of His tagged recombinant malate dehydrogenase (MDH) (unpublished)

Backbone

• Vector backbone: pET24a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5271
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MDH_9CREN
• Species: Ignicoccus islandicus
• Insert Size (bp): 948
• GenBank ID: NZ_CP006867
• Promoter: Lac
• Tag / Fusion Protein:
  • 6X His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • pET24a 9CREN MDH SnapGene
  • MCC Clone Information Sheet - MDH_9CREN

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/A0A0U3FQH7/.

Originally identified in Ignicoccus islandicus, a genus of archaea, living in a hydrothermal vent growing in ~90 °C. This is an NADHP-dependent tetramer with LDH-like activity and may be a divergent homolog from MDH and LDH with different allosteric properties.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.