pET24a MDH_CHLP8
(Plasmid
#204242)
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PurposeBacterial expression of C. parvum (green sulfur photosynthetic bacterium) malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 204242 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET24a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5237
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMDH_CHLP8
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SpeciesChlorobaculum parvum
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Insert Size (bp)969
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GenBank IDNC_011027.1
- Promoter Lac
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Tag
/ Fusion Protein
- 6X His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
UniProt ID: https://www.uniprot.org/uniprotkb/B3QPY9/
For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET24a MDH_CHLP8 was a gift from Malate dehydrogenase CUREs Community (MCC) (Addgene plasmid # 204242 ; http://n2t.net/addgene:204242 ; RRID:Addgene_204242)