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pET24a MDH_STRAW
(Plasmid #204240)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204240 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5237
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MDH_STRAW
  • Species
    Streptomyces avermitilis
  • Insert Size (bp)
    1026
  • GenBank ID
    NZ_JZJK01000090.1
  • Entrez Gene
    mdh (a.k.a. SAV_3436)
  • Promoter Lac
  • Tag / Fusion Protein
    • 6X His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/Q82HS2/.

-Soil bacteria with unique secondary metabolites with anti-helminthic and insecticidal properties.
-Thermostable MDH with a duel NADH and NADPH substrate.
-Strongly inhibited by both OAA and NADH, while other ions increase activity.
-One article published: https://pubmed.ncbi.nlm.nih.gov/20845078/.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.

Plasmid has been found to exist as a dimer. Plasmid dimerization often does not impact plasmid function, but may reduce transformation efficiencies. If you need to isolate the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. Please see our blog post for more information: https://blog.addgene.org/plasmids-101-dimers-and-multimers

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET24a MDH_STRAW was a gift from Malate dehydrogenase CUREs Community (MCC) (Addgene plasmid # 204240 ; http://n2t.net/addgene:204240 ; RRID:Addgene_204240)
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