pET24a MDH_AQUAR
(Plasmid #204239)

• Purpose: Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
• Depositing Lab: Malate dehydrogenase CUREs Community (MCC)
• Publication: Expression of His tagged recombinant malate dehydrogenase (MDH) (unpublished)

Backbone

• Vector backbone: pET24a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5211
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MDH_AQUAR
• Species: Aquaspirillum arcticum
• Insert Size (bp): 1052
• GenBank ID: AF109682.1
• Promoter: Lac
• Tag / Fusion Protein:
  • 6X His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • pET24a MDH AQUAR SnapGene
  • MCC Clone Information Sheet - MDH_AQUAR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/Q9ZF99/.

Bacterium isolated from under snow and ice, in the northwest territory of Canada. The sequence of this MDH is highly related to thermophilic MDH. This protein is 2-3-fold more active at 4-10 °C than other MDH enzymes – possibly due to an increased flexibility at the active site as well as reduced interactions at subunits.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.