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pET24a MDHNP_ARATH
(Plasmid #204238)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5268
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MDHNP_ARATH
  • Alt name
    Arabidopsis chloroplast MDH
  • Alt name
    acMDH2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1197
  • Mutation
    Does not include the chloroplastic transit sequence and includes a MET for start site
  • GenBank ID
    NM_125218.4
  • Entrez Gene
    AT5G58330 (a.k.a. AT5G58330, MCK7.20, MCK7_20, NADP-MDH, NADP-dependent Malate Dehydrogenase)
  • Promoter Lac
  • Tag / Fusion Protein
    • 6X His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/Q8H1E2/.

Plays an essential role in the regulation of catalase activity and the accumulation of a hydrogen peroxide-dependent signal by transmitting the redox state of the chloroplast to other cell compartments.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET24a MDHNP_ARATH was a gift from Malate dehydrogenase CUREs Community (MCC) (Addgene plasmid # 204238 ; http://n2t.net/addgene:204238 ; RRID:Addgene_204238)
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