pET24a MDHC1_ARATH
(Plasmid #204237)

• Purpose: Bacterial expression of Arabidopsis cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
• Depositing Lab: Malate dehydrogenase CUREs Community (MCC)
• Publication: Expression of His tagged recombinant malate dehydrogenase (MDH) (unpublished)

Backbone

• Vector backbone: pET24a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5268
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MDHC1_ARATH
• Alt name: Arabidopsis cytosolic MDH
• Alt name: acMDH1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1017
• GenBank ID: NM_100321.3
• Entrez Gene: AT1G04410 (a.k.a. AT1G04410, F19P19.13, F19P19_13)
• Promoter: Lac
• Tag / Fusion Protein:
  • 6X His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • pET24a MDHC1 ARATH SnapGene
  • MCC Clone Information Sheet - MDHC1_ARATH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/P93819/.

This clone has been optimized for bacterial expression and will not match the plant gene sequence.

The expression or activity of this clone in pET28a has not been tested – includes thrombin but not TEV site between C-term His-tag and MDH.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.