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pET24a MDHM1_ARATH
(Plasmid #204236)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204236 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5271
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MDHM1_ARATH
  • Alt name
    Arabidopsis mitochondrial MDH
  • Alt name
    amMDH2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    978
  • Mutation
    Does done include the first 52 residues that are the mitochondrial targeting peptide. A Met is inserted for the start codon.
  • GenBank ID
    NP_171936.1
  • Entrez Gene
    mMDH1 (a.k.a. AT1G53240, mitochondrial malate dehydrogenase 1)
  • Promoter Lac
  • Tag / Fusion Protein
    • 6X His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/Q9ZP06/.

Required for carbon dioxide and energy partitioning in leaves. May limit photorespiration during the dark phase. Its activity is essential to shuttle reductants out of the mitochondria to support photorespiration.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET24a MDHM1_ARATH was a gift from Malate dehydrogenase CUREs Community (MCC) (Addgene plasmid # 204236 ; http://n2t.net/addgene:204236 ; RRID:Addgene_204236)
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