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pET28a MDH_PFALCI
(Plasmid #204235)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204235 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5235
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MDH_PFALCI
  • Alt name
    MDH PLAF7
  • Alt name
    PfMDH
  • Species
    Plasmodium falciparum
  • Insert Size (bp)
    978
  • GenBank ID
    PF3D7_0618500
  • Entrez Gene
    PF3D7_0618500 (a.k.a. PF3D7_0618500)
  • Promoter Lac
  • Tag / Fusion Protein
    • 6X His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/C6KT25/.

From Plasmodium falciparum (isolate 3D7) – deadliest species of Plasmodium causing malaria in humans.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a MDH_PFALCI was a gift from Malate dehydrogenase CUREs Community (MCC) (Addgene plasmid # 204235 ; http://n2t.net/addgene:204235 ; RRID:Addgene_204235)
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