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pET28a MDHP_YEAST
(Plasmid #204233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5235
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MDHP_YEAST
  • Alt name
    Yeast peroxisomal MDH
  • Alt name
    peroxisomal yeast MDH, Yeast MDH3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1068
  • Mutation
    The last 3 C-terminal residues (SKL) are the peroxisomal transit signal and were left in the final version.
  • GenBank ID
    NM_001180137.1
  • Entrez Gene
    MDH3 (a.k.a. YDL078C)
  • Promoter Lac
  • Tag / Fusion Protein
    • 6X His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/P32419/.

The affinity of MDH3 for OAA is lower than those of MDH1 and MDH2. The structure of the ternary complex suggests that the active-site loop is in the open conformation, differing from the closed conformations in mitochondrial and cytosolic malate dehydrogenases.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a MDHP_YEAST was a gift from Malate dehydrogenase CUREs Community (MCC) (Addgene plasmid # 204233 ; http://n2t.net/addgene:204233 ; RRID:Addgene_204233)
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