pET28a MDHC_YEAST
(Plasmid #204232)

• Purpose: Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
• Depositing Lab: Malate dehydrogenase CUREs Community (MCC)
• Publication: Expression of His tagged recombinant malate dehydrogenase (MDH) (unpublished)

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5237
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MDHC_YEAST
• Alt name: Yeast Cytosolic MDH
• Alt name: Cytoplasmic Yeast MDH2
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1170
• GenBank ID: NM_001183380.1
• Entrez Gene: MDH2 (a.k.a. YOL126C)
• Promoter: Lac
• Tag / Fusion Protein:
  • 6X His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • pET28a-MDHC_YEAST SnapGene
  • MCC Clone Information Sheet - MDHC_YEAST

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/P22133/.

Interacts with PEPCK and Fructose Bisphosphatase 1. This isoform is reported to translocate to nuclear periphery in response to DNA damage.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.